HTS assay development

HTS assay optimisation 

Throughout assay optimisation, we work closely with you to select the ideal buffer conditions (e.g., pH, additives detergents) and parameters (enzyme/substrate concentrations relative to Kd or Km, incubation time). We collaborate with you on your desired mode-of-action as well as validate and refine assay conditions together. Our team discusses, troubleshoots and adapts as needed, carefully characterising enzyme binding sites, substrates and co-factors to achieve in vitro conditions that closely reflect physiological reality. 

Assay automation for HTS compatibility 

Our assay development process for HTS compatibility encompasses a range of services tailored to meet the specific needs of your project. From set-up in 384-well formats to miniaturisation in 1,536-well formats, we ensure that each step is optimised for precision and efficiency. Our approach includes thorough stability testing, evaluations of robustness and high-quality pre-screening to deliver reliable and reproducible results. Our key development and optimisation services include: 

• Substrate, ligand/tracer and interaction partner screening  
• High-density format conversion and miniaturisation 
• Determination of IC50 values for reference compounds 
• Reagent, enzyme and signal stability testing  
• Assay robustness assessment 
• Pre-screening of a diverse in-house 10k compound test library.