Assay Types, Readout Technologies, and Target Classes

Our scientists are well-versed in developing biochemical assays for a wide range of targets and using various readout technologies. We have successfully developed HTS-compatible enzyme activity assays (e.g., kinetic, end-point, single, or coupled enzyme assays), as well as equilibrium-binding assays (e.g., protein–peptide, protein–protein, protein–DNA, protein–RNA). Our scientists are experienced with challenging assay development and will deliver robust, miniaturized, cost-effective, fully optimized, and high-throughput compatible assays to fit the needs of the entire drug discovery process.

Some of our favorite readout technologies:

  • TR-FRET/HTRF (time-resolved fluorescence resonance energy transfer)
  • Luminescence
  • Fluorescence polarization
  • Fluorescence intensity
  • Absorbance
  • AlphaScreen (amplified luminescent proximity homogeneous assay)
  • BRET/nanoBRET (bioluminescence resonance energy transfer)
  • Mass spectrometry readouts (native substrates and products): MALDI MS and LC–MS
  • Radiometric readouts (scintillation proximity assays)
  • Surface plasmon resonance (SPR)
  • Thermal shift assays (TSA)

We have experience with a wide range of target classes, including:

  • acetylases
  • deaminases
  • dehydrogenases
  • deubiquitinating enzymes
  • E3-Ligases
  • exchange factors
  • hydrolases
  • lipases
  • GTPases, ATPases
  • kinases
  • methyltransferases
  • oxygenases
  • phosphatases
  • protein–protein interactions
  • reductases
  • RNA binding
  • RNA modifying

and many more.

If you are interested in more details about specific steps during the development of screening assays and possible hit characterization assays, see the following: