Microscale thermophoresis

Analysing binding affinities and molecular interactions

MST measures the migration of a fluorescence-labeled molecule in a temperature gradient, which depends on the molecule's size, charge and hydration shell. Changes in these parameters indicate binding events, making MST a powerful tool for determining interactions. Our use of a Monolith NTAutomated instrument can determine binding affinities ranging from nanomolar to millimolar, supporting screening projects effectively. We also use MST to cross-validate SPR results and support mode-of-action studies. Our expertise includes characterising protein–ligand, protein–RNA, protein–DNA and RNA–ligand interactions. For protein–ligand interactions, we use specific labelling methods, while for protein–RNA, protein–DNA and RNA–ligand interactions, we utilise commercially available fluorescence-labeled RNA and DNA, requiring no additional labelling.