Isochronic design is a methodological approach used to assess the stability of biomarkers or other analytes over time by leveraging the assumption that their levels remain stable and unchanged at very low temperatures, typically -150°C.
Aliquots of a sample are stored at -150°C to serve as the baseline or "unstressed" condition. At the same time, other aliquots of the same sample are stored at higher, "stressed" temperatures such as -75°C and -20°C. After a designated storage period the stressed samples are transferred to -150°C. This step essentially "freezes" the state of the sample at that specific time point, preserving the condition it was in after being stored at the stressed temperature. For stability measurement, both unstressed and stressed samples are measured in the same analytical run and compared to evaluate the stability of the biomarker.
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