Extending from 2D cell culture models, we are leading experts in establishing meaningful 3D cell culture based high-content assays that better reflect tissue or disease physiology. These assays encompass multicellular tumor spheroids or organoids and can be used to study cell invasion, cell migration, or cell–tissue interactions. Crucially, we have the know-how to make these assays high-throughput compatible, having successfully conducted screening campaigns with more than 400,000 compounds on 3D models.

3D brain organoids stained for Hoechst, neuronal, and stem cell markers.

Our scientists have developed 3D co-cultures that can be used as a phenotypic HCI screening system to study invasive processes in multicellular tumor models. We are one of the few screening laboratories in the world that can measure, analyze, and quantify invasion processes in high-throughput phenotypic models to test anti-invasive agents in vitro.

Our scientists have successfully adapted the organoid technology for a range of indications to be handled in 384-well plates, making them HTS-compatible. Using our advanced image analysis algorithms, we can identify agents that significantly prevent the development of a disease-relevant phenotype.

Kidney oganoid in 384-well plate format to model polycystic kidney disease.

We established the following 3D HCA assays:

  • Organoids HCA

    • Multiplexed, HTS-compatible
    • Evaluation of organoid biology
    • Assay development ongoing for several indication

      High-throughput capable iPSC derived organoids grown on 384W plates for > 6 weeks. Organoids are viable as shown by Hoechst (blue) and SYTOX green (green) staining.