Metabolite discovery

Metabolite discovery and analysis

Our platform provides end-to-end support for drug metabolite discovery, synthesis, purification and analysis.

Metabolite screening and synthesis

• Screening: use of wild-type fungi, bacteria strains, human and bacterial CYPs in cells and isolated enzymes
• Synthesis: late-stage diversification via chemical oxidations and glucuronidations
• De novo synthesis: combining classical methods with modern techniques (photo- and electrochemistry).

Fermentation and scale-up 

• Fermentation platforms:
  • 1–2 L Biostat B-DCU for optimisation
  • 5–100 L steel fermenters for scale-up
• Generation of drug metabolites in milligram to gram scale via biotransformation.

Purification and analytics

• State-of-the-art chromatography (normal, reversed-phase, supercritical fluid)
• Chiral separations and screening (up to 200 combinations)
• Characterisation via NMR, HPLC, HRMS and UPLC-MS
• Stability evaluations and physicochemical property measurement (logD, solubility, pKa).

DMPK support

• Metabolite identification in biological matrices (in vitro, in vivo)
• Metabolic stability, permeability and protein binding assessments