Metabolite discovery and analysis
Our platform provides end-to-end support for drug metabolite discovery, synthesis, purification and analysis.
Metabolite screening and synthesis
- Screening: use of wild-type fungi, bacteria strains, human and bacterial CYPs in cells and isolated enzymes
- Synthesis: late-stage diversification via chemical oxidations and glucuronidations
- De novo synthesis: combining classical methods with modern techniques (photo- and electrochemistry).
Fermentation and scale-up
- Fermentation platforms:
- 1–2 L Biostat B-DCU for optimisation
- 5–100 L steel fermenters for scale-up
- Generation of drug metabolites in milligram to gram scale via biotransformation.
Purification and analytics
- State-of-the-art chromatography (normal, reversed-phase, supercritical fluid)
- Chiral separations and screening (up to 200 combinations)
- Characterisation via NMR, HPLC, HRMS and UPLC-MS
- Stability evaluations and physicochemical property measurement (logD, solubility, pKa).
DMPK support
- Metabolite identification in biological matrices (in vitro, in vivo)
- Metabolic stability, permeability and protein binding assessments
