Ligand Binding & Flow Cytometry Assays

LIGANDBINDING 
ASSAYS PARARMETER

ASSAY
ESTABLISHED

ASSAY
QUALIFIED

ASSAY
VALIDATED

ADMA

 

 

x

Adrenaline

x

 

 

AKT (phospho AKT)

 

 

x

AKT (total AKT)

 

 

x

Aldosterone

 

 

x

Alpha 1 Acid Glycoprotein

x

 

 

Apolipoprotein B-48

x

 

 

CK 18

x

 

 

C-peptide

x

 

 

CRP

x

 

 

C-terminal Propeptide
Type I Collagen (CICP CTX)

 

x

 

Dihydrotestosterone

x

 

 

Eotaxin

 

 

x

Eotaxin-3

 

 

x

Erythropoietin

 

 

x

FGF-23

 

 

x

G-CSF

 

 

x

GM-CSF

 

 

x

Glucagon

 

x

 

GLP-1

 

x

 

GLP-2

 

x

 

Hemoglobin

x

 

 

IFN-α

 

x

 

IFN-γ

 

 

x

IGF-1

 

x

 

IL-1α

 

 

x

IL-1β

 

 

x

IL-2

 

 

x

IL-4

 

 

x

IL-5

 

 

x

IL-6

 

 

x

IL-7

 

 

x

IL-8

 

 

x

IL-10

 

 

x

IL-12p70 x

 

 

 

IL-12/IL-23p40

 

 

x

IL-13

 

 

x

IL-15

 

 

x

IL-16

 

 

x

IL-17A

 

 

x

IL-23

 

x

 

Inhibin B

x

 

 

Insulin

 

x

 

IP-10

 

 

x

JAK2 (phospho JAK2)

x

 

 

JAK2 (total JAK2)

x

 

 

Leuprolide

 

 

x

LTB4

 

x

 

M30 Apoptosome

 

x

 

M65 Apoptosome

 

x

 

MCP-1

 

 

x

MCP-4

 

 

x

FSH

 

 

x

LH

x

 

 

MDC

 

 

x

β2-microglobulin

 

x

 

MIP-1α

 

 

x

MIP-1β

 

 

x

Noradrenaline

x

 

 

Octreotide

 

 

x

P1NP

 

x

 

PAI-1

x

 

 

PRAS40 (phospho PRAS40)

 

 

x

PRAS40 (total PRAS40)

 

 

x

Procalcitonin

 

 

x

p-Selectin

x

 

 

PSA

 

 

x

sEGFR

x

 

 

SHBG

 

 

x

sIL-2R

x

 

 

STAT3 (phospho STAT3)

x

 

 

STAT3 (total STAT3)

x

 

 

STAT5 (phospho STAT5)

x

 

 

STAT5 (total STAT5)

x

 

 

sTNF-RI

x

 

 

sTNF-RII

x

 

 

TARC

 

 

x

TGF-β1

 

x

 

TNF-α

 

 

x

TNF-β

 

 

x

Total T3

 

 

x

Total T4

 

 

x

VEGF

 

 

x

 

FLOW CYTOMETRYASSAYS

CD3

 

 

x

CD4

 

 

x

CD8

 

 

x

CD11

x

 

 

CD11b

x

 

 

CD14

 

x

 

CD19

 

x

 

CD25

 

 

x

CD28

 

 

x

CD34

 

x

 

CD38

x

 

 

CD40

x

 

 

CD45

x

 

 

CD45RO

 

 

x

CD49b

x

 

 

CD69

x

 

 

CD314

x

 

 

CFSE (proliferation assay)

 

 

x

FoxP3

 

 

x

Ly-6C

x

 

 

Ly-6G

x

 

 

 

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